Background: Immune responses is activated by the production of cytokines and the bacterial components of the primary immune cells such as macrophages that control expression of the genes in chromosome. So, we generated B. abortus mutants based on transposon mutation for investigation of immune responses. Five mutants were selected by different growth rates. In this study, the productions of inflammatory mediator, cytokines, and activation of toll-like receptors (TLR1 to TLR9) were investigated after stimulation of the mutants in RAW 264.7 cells. Their correlation between the growth rates and such productions were analyzed. Methods: The productions of nitric oxide (NO) and the cytokines were quantified by NO assay and ELISA. Expressions of TLRs were analyzed by real-time PCR. Results: NO and cytokines were highly produced after stimulation of B. abortus mutants compared to the wild type in dose and time dependent manner. Production levels of TNF-α, IL-1β, and IL-6 were highly increased compared to other cytokines after the stimulation with the MOI 100 of the B. mutants. Especially, stimulation of C3 mutant showed low productions of NO, IL-6, and TNF-α compared to wild type at 24 h, whereas C13, which showed similar growth rate with C3, highly produced these cytokines. However, productions of IL-12p40 and IFN-γ were not detected. Activations of TLRs showed different expression patterns in each dose dependent stimulations of the mutants. Conclusion: Our results suggest that immune responses were related to the growth rates of the mutants that showed different production levels of cytokines. However, C13 and C24 mutants were exceptional to such correlation. Therefore, analysis of gene expression profiles are needed as the further study. This study was supported by NRF (No. 2014R1A2A2A01007291), the BK21 PLUS program for Creative Veterinary Science Research, and the Research Institute of Veterinary Science, Seoul National University, Republic of Korea.