Poster Presentation International Veterinary Immunology Symposium 2016

Porcine T-cell responses in peripheral blood mononuclear cells infected with porcine reproductive and respiratory syndrome virus (#129)

seong-hee kim 1 , kyoung-ki lee 1 , yeon-hee kim 1 , byeongjae so 1 , ji-young park 1
  1. Animal and plant quarantine agency, Gimcheon-si, GYEONGSANGBUK-DO, South Korea

The rapid generation of genetic variation in porcine reproductive and respiratory syndrome virus (PRRSV) makes it difficult to characterize the porcine T-cell response to this virus. We evaluated the dynamic fluctuations of porcine T-cell responses in peripheral blood mononuclear cells (PBMCs) infected with North American PRRSV (strain 42) followed by inoculating PRRSVs with high- (strains 383 and LMY; Group 1) and/or low-potency (strain 415 and 380; Group 2) neutralizing antibodies for strain 42. The relative numbers of T cells were determined two, six, and eight hours post-inoculation (hpi) by flow cytometry and cytokines (IL-2, IL-6, and IL-10) were examined by enzyme-linked immunosorbent assay. Populations of CD4+, CD4, and CD8cells were not significantly different upon infection with Group 1 and 2 viruses in PBMCs. However, the frequency of CD8+ and CD3+ cells slightly increased eight hpi with Group 1 viruses as well as with strain 42. Those cells also produced more IFN-γ and TNF-α, and IL-10 secretion was significantly downregulated in cell supernatant infected with Group 1 viruses compared with Group 2 viruses. However, each PRRSV did not influence the level of other cytokines (IL-6 and IL-12). CD3 expression analysis suggested stronger T-cell activation in Group 1 infection. This study shows that serum neutralizing antibody titer among viruses is related to certain cytokines, suggesting that those cytokines could contribute to customized vaccine development.

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  2. 2 Ferrari L, Martelli P, Saleri R, et al.: 2013, Lymphocyte activation as cytokine gene expression and secretion is related to the porcine reproductive and respiratory syndrome virus (PRRSV) isolate after in vitro homologous and heterologous recall of peripheral blood mononuclear cells (PBMC) from pigs vaccinated and exposed to natural infection. Vet Immunol Immunopathol 151:193-206.